Contractile Activities of Synthetic Bradykinin Fragments on Guinea Pig Ileum

Effect of aqueous fraction of Rosa damascena on ileum contractile response of guinea pigs

Objective: The use of drugs with herbal origin is increasing for treatment of gastrointestinal (GI) disorders. Rosa damascena (R. damascena) is a well-known plant suggested to have beneficial effect on GI system. In this study, the effect of aqueous fraction of R. damascena on the contractionsofisolated guinea pig ileum was investigated. Materials and Methods: Aqueous fraction of plant was obta...

Effect of morphine and acetylcholine on contractile activity and cyclic AMP in guinea-pig ileum.

Neither acute nor prolonged exposure to morphine altered cAMP content or spontaneous movements of longitudinal muscle-myenteric plexus strips of the guinea-pig ileum. By contrast, exogenous acetylcholine or electrical stimulation of the strips elicited both a decrease of cAMP concentration and a twitch response. Atropine blocked the effects of stimulation on these parameters. Addition of morphi...

Potentiation of the biphasic bradykinin response of the guinea-pig ileum.

Biologically active synthetic fragments of bradykinin.

Bradykinin, Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, is released from bradykininogen by the action of kallikreins and brings about smooth muscle stimulation, vasodilation, increase in capillary permeability, ac cumulation and migration of leucocytes and pain production. It is well known that so-called kininase, for example catheptic carboxypeptidase B and carboxypeptidase N, destroy bradykinin by c...

Heterogeneous contractile responses of single smooth muscle cells from guinea pig ileum.

Cells of guinea pig ileal longitudinal muscle layer were dispersed by treating the minced tissue with highly purified collagenase and papain. The cells were spindle shaped, 100 to 150 microns long and 5 to 10 microns wide. Contractility of the dispersed cells was determined by "cell adhesion method" resulting that the cells were contracted by carbachol in a dose-dependent manner, but sensitivit...